Ahasteen-Bryant was born and raised on the Navajo reservation in New Mexico. She is from Diné (Navajo) Nation. Her maternal clan is Naashashi' (Bear) clan, and she was born for the Kinyaa'áanii (Towering House People) clan, also known as her paternal clan. She received her bachelor's degree in business administration from New Mexico State University in 1989 and her master's degree in secondary education from Indiana University. In 2009, she was appointed as director of the Native American Educational and Culural Center at Purdue University and currently holds that position. In March 2007, she joined the Purdue College of Agriculture with the role of program coordinator in the office of multicultural programs.